link.springer.com/article/10.1002/j.1460-2075.1990.tb07459.xPDF

Initial steps in protein membrane insertion. Bacteriophage M13 procoat protein binds to the membrane surface by electrostatic interaction.

… and chased with an excess of non-radioactive methionine (Figure 2B). At different chase times, … the same features of membrane insertion and processing within a chase time of 10 min. …

www.jbc.org/article/S0021-9258(19)77075-6/fulltextPDF

Function of YidC for the insertion of M13 procoat protein inEscherichia coli: Translocation of mutants that show differences in their membrane potential dependence …

… For the Procoat H5 study, the cells were pulse-labeled with [ 35 S]methionine for 1 min and chased for 5 min. For PCLep, the cells were labeled for 30 s and chased for 2 min. After …

The Ecclesiology of Donald Robinson and D

academic.oup.com/plphys/article-abstract/118/1/59/6085616PDF

The H+-Sucrose Cotransporter NtSUT1 Is Essential for Sugar Export from Tobacco Leaves

… Autoradiographs of leaves show a heterogenous pattern of CO 2 fixation even after a 24-h chase. The 14 C pattern does not change with time, suggesting that movement of …

europepmc.org/article/med/1923499

Phosphorylation and rapid turnover of hepatitis B virus X-protein expressed in HepG2 cells from a recombinant vaccinia virus.

… In pulse-chase experiments, X-protein decayed with a bimodal half-life of 15 min and 3 h. X-protein having a half-life of about 15 min was found associated with the Triton X-100 …

onlinelibrary.wiley.com/doi/abs/10.1046/j.1365-2958.1997.3591731.xPDF

Isolation and characterization of the mycobacterial phagosome: segregation from the endosomal/lysosomal pathway

… bovis BCG after 1 h of infection and 3 h of chase. M. bovis BCG cells were cultivated in … 5B) was found in phagosomes after 1 h of infection or after a 13h chase. This indicates that at …

www.jbc.org/article/S0021-9258(19)30822-1/fulltextPDF

A Noc complex specifically involved in the formation and nuclear export of ribosomal 40 S subunits

… After 3 min the chase was started by adding unlabeled uracil to a final concentration of 200 μg/ml. Samples of 1 ml were frozen in liquid nitrogen just after starting the chase at the time …

Lead white

www.jbc.org/article/S0021-9258(19)36354-9/fulltextPDF

Direct interaction of YidC with the Sec-independent Pf3 coat protein during its membrane protein insertion

YidC is a newly defined translocase component that mediates the insertion of proteins into the membrane bilayer. How YidC functions in the insertion process is not known. In this study, …

febs.onlinelibrary.wiley.com/doi/abs/10.1111/j.1432-1033.1994.00891.xPDF

Evidence for a Loop‐Like Insertion Mechanism of Pro‐Omp A into the Inner Membrane of Escherichia Coli

We have studied the insertion of pro‐OmpA into the Escherichia coli membrane in vivo using various mutants that have either alterations in the amino‐terminal parts of the signal …