… onine for 2 min and then chased with non-radioactive methionine for 3 min. In the no-azide study, cells were pulse-labeled for 2 min and chased with non-radioactive methionine for 3 …
… This explanation is unlikely, however, because after a long chase period (24 hr), all of the viral … , whether such a long chase is necessary or whether after a short chase at very late times …
… The cells were then chased by adding … chase). At each 1 hour-interval, BCSFB culture inserts were removed and the samples of the donor (basolateral chase) and receiver (apical chase…
… Each of these mutant procoats rapidly chased to its … with "S-methionine for 30s and chased with an excess of L-methionine for the … Twelve such clones were investigated by pulse-chase …
… [3H]Phorbol 12,13-dibutyrate (PDB) (17.5 Ci/mmol; 1 Ci = 3.7 x 1010becquerels) was purchased from New England Nuclear. The stock solution was diluted to 1 AMin 20 nM Tris HCI, …
PURPOSE/HYPOTHESIS: Femoroacetabular impingement syndrome (FAIS) is commonly associated with significant physical activity limitations and decreased daily function in young, …
… Chase holds a Bachelor of Science in electrical engineering from Utah State University. … Richard Kuhn is a computer scientist in the Computer Security Division of NIST. His current …
… Y516S) had sufficient activity to convert most of PC-Lep in the chase, we performed a pulse-chase experiment. In the pulse-chase experiments for F433S, L434S, and Y516S, PC-Lep …
… Since our study is based on pulse-chase experiments, the fast kinetics of membrane targeting is better resolved. Also, a GFP-SciP fusion protein was inhibited to form a membrane …
We introduce a high-affinity split-HaloTag comprised of a short peptide tag (Hpep, 14 residues) and a large, inactive fragment (cpHaloΔ3). Hpep binds to cpHaloΔ3 spontaneously with …